The increasing use of English as a Medium of Instruction (EMI) in schools and higher education institutions in multilingual countries like India has led to significant sociolinguistic changes. While EMI offers access to global opportunities and improves employability, it also presents a challenge to the survival and growth of regional languages. This study investigates the impact of EMI on regional languages, with a focus on language usage patterns, student and teacher perceptions, and institutional language policies. Quantitative and qualitative methods were employed, involving surveys, interviews, and document analysis. The findings reveal a noticeable shift in linguistic preferences, reduction in fluency in regional languages, and limited use of native languages in academic and social domains. The paper concludes with suggestions to ensure linguistic balance and preserve cultural identity.